Microfluidic-Enabled Multi-Cell-Densities-Patterning and Culture Device for Characterization of Yeast Strains’ Growth Rates under Mating Pheromone

Yeast studies usually focus on exploring diversity in terms of a specific trait (such as growth rate, antibiotic resistance, or fertility) among extensive strains. Microfluidic chips improve these biological manner high throughput and efficiency. For population study yeast, it is great significance to set proper initial cell density for every strain under circumstances. Herein, we introduced novel design chip, which enables users load cells gradient order (six alternatives) within one channel. We discussed several guidelines choose the appropriate chamber ensure successful data recording. With this successfully studied rate yeast strains mating response, crucial yeasts control behaviors prosperous mating. investigated eight different three pheromone levels (0.3 ?M, 1 10 ?M). Strains with, even, six-fold can be recorded, with available produced simultaneously. This work has provided an efficient time-saving microfluidic platform, loading pattern multi-cell densities experiment, especially high-throughput study. Besides, quantitatively analyzed shall reveal inspiring perspectives concerning behavior stimulated pheromone.